科创中国

Aux/IAA基因能够在生长素诱导的早期做出响应.本研究通过筛选花生未成熟种子的cDNA文库,分离并获得了4个可能的Aux/IAA基因,分别命名为PNIAA1、PNIAA2、PNIAA3和PNIAA4.RT-PCR对表达模式的研究显示,四个基因在花生各组织中为组成型表达,且具有不同的时空表达模式和特征,PNIAA1、PNIAA2和PNIAA4主要在叶中表达,而PNIAA3在种子中表达量较高,推测其可能在种子发育过程中行使功能;IAA对花生诱导表达模式分析显示,四个基因经IAA诱导后在根部的表达模式均有变化,其中PNIAA1、PNIAA2和PNIAA3能被生长素迅速诱导.

在大田条件下,研究砂姜黑土和砂壤土两种类型土壤对旱地花生植株的生理特性及农艺性状的影响,结果表明:生育期内两类土壤上花生叶片的叶绿素含量、抗衰老酶活性(SOD、POD、CAT)、可溶性蛋白含量及叶面积系数均呈单峰曲线,峰值出现在出苗后60~75d.砂姜黑土花生叶绿素和可溶性蛋白含量及叶面积系数高于砂壤土,而抗衰老酶活性和丙二醛(MDA)含量低于砂壤土.砂姜黑土花生叶面积系数和干物质积累速率的峰值持续时间明显高于砂壤土.砂姜黑土花生的主茎高、侧枝长及单株结果数等主要农艺性状明显优于砂壤土(p

为明确78%扑·噻·乙草胺悬乳剂防除花生田杂草的效果和对花生的安全性,采用田间小区试验的方法,观察其对花生田杂草的防效和对花生产量的影响.结果表明,78%扑·噻·乙草胺悬乳剂有效成分用量1170.0~1521.0g/hm2,药后20 d和40 d对花生田杂草总体的株防效和鲜重防效均在90%以上,并对花生安全,增产率14.87%~16.15%.

Pocket?gophers?(Geomyidae)?have?an?extensive?impact?on?both?natural?and?agricultural?systems.?Trapping?is?a?tech-?nique?often?used?to?sample?and?manage?these?populations.?The?identification?of?an?attractant?that?increases?capture?rates?of?pocket?gophers?would?greatly?assist?researchers?and?pest?management?professionals?alike.?Therefore,?we?tested?the?attractiveness?of?four?attractants?(peanut?butter,?anise,?grapefruit?attractant,?and?carrot)?plus?a?control?(no?attractant)?using?uncovered?and?covered?trap?sets?to?determine?what?impact?they?have?on?visitation?and?capture?rates?of?pocket?gophers.?We?also?determined?how?the?impact?of?cover?status?and?attractants?differ?across?varying?weights?and?gender?of?pocket?gophers.?We?found?no?direct?effect?of?any?attracrant?on?visitation?and?capture?rates?of?pocket?gophers,?nor?on?the?gender?of?captured?individuals.?However,?when?no?attractant?was?used,?the?mtmber?of?pocket?gophers?captured?per?100?trap?nights?was?greater?when?trap?sets?were?uncovered?vs.?when?covered,?and?capture?rates?were?generally?high?and?consistent?when?using?peanut?butter?as?an?attractant.?Additionally,?we?noted?that?covered?trap?sets?that?were?baited?with?peanut?butter?yielded?heavier?pocket?gopher?captures?than?uncovered?trap?sets?using?this?same?attractant.?This?is?key,?given?the?difficulty?associated?with?capturing?older,?more?experienced?individuals.?Combined?with?data?from?a?previous?investigation,?this?suggests?that?there?is?no?advantage?to?using?any?attractant?when?utilizing?uncovered?trap?sets,?but?there?is?likely?some?benefit?to?using?peanut?butter?in?covered?trap?sets?[Current?Zoology?60?(4):?472-478,?2014].

目的 观察5-脂氧合酶(5-LOX)对缺氧诱导的视网膜新生血管形成的抑制作用,探讨视网膜新生血管形成的可能机制.方法 鼠龄为7d的C57BL/6J幼鼠随机分为正常组、氧诱导视网膜病变(OIR)模型组、大剂量干预组、小剂量干预组、干预对照组.各组分别为12只.除正常组外,其余各组小鼠与哺乳母鼠共同置于氧浓度为(75±2)%的氧箱内饲养5d后转移至正常环境中饲养5d,建立OIR模型.12~17日龄时,大剂量干预组、小剂量干预组小鼠腹腔每天分别注射100、50 mg/kg 5-LOX抑制剂去甲二氢愈创木酸;干预对照组小鼠腹腔每天注射等体积1%二甲基亚砜;OIR模型组小鼠出氧箱后不作任何处理.石蜡切片苏木精-伊红染色并计数突破视网膜内界膜的血管内皮细胞核,CD34标记鼠新生血管内皮细胞,逆转录-聚合酶链反应(RT PCR)检测视网膜中5-LOX、血管内皮生长因子(VEGF)-a、VEGF受体-2(VEGFR-2)的mRNA含量.蛋白质免疫印迹法(Western blot)检测视网膜中5-LOX、VEGF-a、VEGFR-2、细胞外磷酸化信号调节蛋白激酶(P-ERK) 1/2的蛋白表达含量.结果 不同剂量干预组突破内界膜的视网膜新生血管内皮细胞核数较OIR模型组、干预对照组明显减少,差异有统计学意义(F=73.390,P<0.05).RT-PCR、Western blot检测结果显示,不同剂量干预组小鼠视网膜5-LOX、VEGF-a、VEGFR-2的mRNA和蛋白表达均较OIR模型组、干预对照组减少,差异有统计学意义(F=92.668,P<0.05);大剂量干预、小剂量干预组之间VEGFR-2蛋白相对表达量比较,差异无统计学意义(F=2.118,P>0.05);5-LOX、VEGF-a、P-ERK 1/2相对蛋白表达比较,差异均有统计学意义(F=86.490、165.128、139.424,P<0.05).结论 缺氧可以诱导视网膜中5-LOX的高表达;5 LOX选择性抑制可以降低其表达并减少缺氧诱导的新生血管形成.