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Ad/Ds系统及其在油菜突变体库构建中的应用策略
随着油菜基因组测序上作的完成和后续完善,油菜功能基因组学必将成为研究热点,如何构建高通量的突变体库则是影响其进展的关键.Ad/Ds(Activator/Dissociation)系统是构建插入型突变体库的理想技术,在水稻(Olyza Sativa)、拟南芥(Arabidopsis thaliana)等异源植物中应用较广,但在油菜中尚处于起步阶段.文章介绍了Ad/Ds系统的结构特点、转座机理等基本概况,阐述了利用Ad/Ds系统构建植物突变体库的优势及存在的问题.结合模式植物水稻和拟南芥的研究成果,进一步分析了利用Ae/Ds系统构建油菜突变体库的改进策略,并对其在油菜功能基因组研究中的应用前景进行了展望.洪林,闫晓红,刘正富,魏文辉 - 南方农业学报文章来源: 万方数据 -
基于射频技术的无线环境监测系统设计与实现
随着射频技术的日益成熟,文中提出了一种无线的环境监测系统.本系统分为1个主机和2个从机,从机利用传感器采集现场房间温度、湿度和空气污染程度等环境参数,利用LCD12864实时显示,并通过nRF905无线通信模块发送采集的环境参数.在主控室主机和计算机终端串口相连,在计算机上通过监测软件实时显示各个房间的各种环境参数,并且还具有报警功能.该系统性能稳定,抗干扰能力强,搭建方便,在现实生活中有很强的实用性.刘燎原,袁德明 - 电子设计工程文章来源: 万方数据 -
基于虚拟现实的室内地震避险教育系统的研究
地震避险知识教育具有重要意义,利用虚拟现实技术模拟地震发生时人的避险体验,比文字宣传更具有视觉、听觉上的冲击力,而相比现实生活中真实的地震避险演练而言可节省更多的宣传成本,收到更好的宣传效果.使用3Ds Max对室内场景和人物角色进行建模,基于Virtools平台进行设计,开发了集声音、动画和人机交互于一体、具有游戏风格界面的室内地震避险教育系统.初步的实验结果表明,该系统能够使地震避险演练变得更加形象,可为地震避险教育宣传提供一种新的手段.胡春花,陈晓梅 - 系统仿真学报文章来源: 万方数据 -
As the popularization of dual ring fieldbus,the optimized dual ring synchronization methods are still in short.The current synchronization methods are generally established in traditional industrial fieldbus,in which transmission is commonly considered in single track,the two-way transmitting cannot take full effect,and would result in unwanted idle load on equipment lines.In stamp-transferring part,the synchronizing algorithm is not properly processed to diminish the latency,so the real-time performance of entire system cannot be ensured.To support the synchronization control of stations in the CNC system,a real-time time synchronization method for dual ring fieldbus in the CNC system is designed in this paper.In this method a synchronizing message transmission scheme based on dual ring architecture and the synchronization algorithm between master and secondary stations are integrated.In the scheme,the clock models of both master and secondary stations are optimized with corresponding modules and the stamp data transmission based on the dual ring fieldbus is devised exclusively,so the transmitting efficiency improves with less idle work.In the algorithm,all the secondary stations can accomplish the consistent state with master station by updating clock discrepancy information in one communication cycle,and it takes the advantage of two-way transmitting and makes the best use of dual ring structure,so the real-time performance of the system can be promoted while retaining the precision of synchronization.To evaluate the performance,the costs of the method and errors during synchronizing are noted and analyzed based on the actual running environment in the industrial fieldbus.The results show that it reduces communication cost and ensures the smoothness of the system with low lag effects under heavy load.The proposed time synchronization method optimizes the architecture of sync message transmission in dual ring fieldbus,and improves the efficiency of time synchronization in the stations of CNC system.- 中国机械工程学报文章来源: 万方数据
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基于单总线数字温度传感器DS18B20的测温系统设计
设计了一种基于单总线数字温度传感器DS18B20的测温系统,给出了DS18B20传感器特性和控制命令及时序,在Proteus环境下进行了测温系统硬件设计,同时采用Keil软件完成了系统主程序、DS18B20驱动程序的设计;最后进行了系统Proteus仿真,提高了系统开发效率,并获得了良好的仿真实验结果.吕建波 - 现代电子技术文章来源: 万方数据 -
美国税制的借鉴和启示
作为市场经济体制比较完善的西方发达国家,美国较为完善的税制体系及税务管理体系,对我国的税制改革和征管改革有很多有益的借鉴.本文通过对美国税制、主要税种的介绍,美国税制特征的分析,提出我国税制结构设计以及改进所得税、财产税、社会保障税等方面的启示.- 涉外税务文章来源: 万方数据 -
日本节能理念和制度创意对我国的启示
"十二五"时期我国节能工作面临着更加复杂的形势和严峻的挑战,需要在以往工作基础上有所创新和突破.日本是全球能源效率最高的国家之一,节能取得卓越成绩不仅依靠对技术的不懈追求,更依靠对节能更加深入的理解和政策上的大胆尝试.通过将节能融入经济发展战略、"以人为本"的管理手段以及用更加系统整体的眼光重新看待节能技术和产品,加上能源管理师、领跑者等制度上的创新,日本一直保持着全球节能引领者地位.本文简要地回顾和总结了日本近年来节能理念和制度方面的创新,并结合我国"十一五"以来节能工作,提出了针对"十二五"节能工作的政策建议.符冠云,郁聪 - 中国能源文章来源: 万方数据 -
足细胞与机体免疫系统
足细胞,作为肾小球滤过屏障的一道重要防线,被认为是蛋白尿产生的中心环节,因此近年来备受关注.免疫抑制剂对足细胞损伤的保护作用促使人们寻找除其免疫抑制作用之外对足细胞直接的保护机制.足细胞本身是否具有免疫细胞样的特性?在病理情况下,足细胞又是如何对机体炎症反应作出应答的?上述问题的解答将对足细胞损伤机制研究和探寻治疗干预措施具有重要意义.郑春霞,刘志红 - 肾脏病与透析肾移植杂志文章来源: 万方数据 -
The existing research of the active suspension system(ASS) mainly focuses on the different evaluation indexes and control strategies. Among the different components, the nonlinear characteristics of practical systems and control are usually not considered for vehicle lateral dynamics. But the vehicle model has some shortages on tyre model with side-slip angle, road adhesion coefficient, vertical load and velocity. In this paper, the nonlinear dynamic model of lateral system is considered and also the adaptive neural network of tire is introduced. By nonlinear analysis methods, such as the bifurcation diagram and Lyapunov exponent, it has shown that the lateral dynamics exhibits complicated motions with the forward speed. Then, a fuzzy control method is applied to the lateral system aiming to convert chaos into periodic motion using the linear-state feedback of an available lateral force with changing tire load. Finally, the rapid control prototyping is built to conduct the real vehicle test. By comparison of time response diagram, phase portraits and Lyapunov exponents at different work conditions, the results on step input and S-shaped road indicate that the slip angle and yaw velocity of lateral dynamics enter into stable domain and the results of test are consistent to the simulation and verified the correctness of simulation. And the Lyapunov exponents of the closed-loop system are becoming from positive to negative. This research proposes a fuzzy control method which has sufficient suppress chaotic motions as an effective active suspension system.- 中国机械工程学报文章来源: 万方数据

