科创中国

目的 研究艾滋病(AIDS)患者高效抗反转录病毒治疗(HAART)后对血脂水平的影响.方法 回顾性调查2005年6月~2011年5月在我科门诊应用高效抗反转录病毒治疗的HIV/AIDS患者100例,平均抗病毒治疗时间(40.78±20.09)个月,HAART方案分别为AZT+ 3TC+ NVP、AZT+ 3TC+ EFV、D4T+ 3TC+ NVP和D4T+ 3TC+ EFV4种,其中D4T+ 3TC+ NVP方案组中体重>60 kg者,D4T的剂量为每次40 mg,每日2次;体重<60 kg者,D4T的剂量为每次30 mg,每日2次.对4组患者治疗前后CD4淋巴细胞计数、空腹血脂(包括总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇)进行比较.结果 HAART后,CD4淋巴细胞计数均升高,总胆固醇、三酰甘油上升,高密度脂蛋白、低密度脂蛋白正常,AZT方案组与D4T方案组比较差异有统计学意义(P<0.05);其中D4T +3TC+ NVP方案组中,体重>60kg、D4T的剂量为每次40 mg与体重<60 kg、D4T的剂量为每次30 mg比较差异有统计学意义(P<0.05);静脉吸毒与性传播所致艾滋病感染,对血脂无明显影响.结论 HAART治疗对5组患者均有效,以D4T为主的HAART治疗方案可引起血脂代谢异常.

The existing research of the active suspension system(ASS) mainly focuses on the different evaluation indexes and control strategies. Among the different components, the nonlinear characteristics of practical systems and control are usually not considered for vehicle lateral dynamics. But the vehicle model has some shortages on tyre model with side-slip angle, road adhesion coefficient, vertical load and velocity. In this paper, the nonlinear dynamic model of lateral system is considered and also the adaptive neural network of tire is introduced. By nonlinear analysis methods, such as the bifurcation diagram and Lyapunov exponent, it has shown that the lateral dynamics exhibits complicated motions with the forward speed. Then, a fuzzy control method is applied to the lateral system aiming to convert chaos into periodic motion using the linear-state feedback of an available lateral force with changing tire load. Finally, the rapid control prototyping is built to conduct the real vehicle test. By comparison of time response diagram, phase portraits and Lyapunov exponents at different work conditions, the results on step input and S-shaped road indicate that the slip angle and yaw velocity of lateral dynamics enter into stable domain and the results of test are consistent to the simulation and verified the correctness of simulation. And the Lyapunov exponents of the closed-loop system are becoming from positive to negative. This research proposes a fuzzy control method which has sufficient suppress chaotic motions as an effective active suspension system.

In the prediction of active vibration isolation performance,control force requirements were ignored in previous work.This may limit the realization of theoretically predicted isolation performance if control force of large magnitude cannot be supplied by actuators.The behavior of a feed-forward active isolation system subjected to actuator output constraints is investigated.Distributed parameter models are developed to analyze the system response,and to produce a transfer matrix for the design of an integrated passive-active isolation system.Cost functions comprising a combination of the vibration transmission energy and the sum of the squared control forces are proposed.The example system considered is a rigid body connected to a simply supported plate via two passive-active isolation mounts.Vertical and transverse forces as well as a rotational moment are applied at the rigid body,and resonances excited in elastic mounts and the supporting plate are analyzed.The overall isolation performance is evaluated by numerical simulation.The simulation results are then compared with those obtained using unconstrained control strategies.In addition,the effects of waves in elastic mounts are analyzed.It is shown that the control strategies which rely on unconstrained actuator outputs may give substantial power transmission reductions over a wide frequency range,but also require large control force amplitudes to control excited vibration modes of the system.Expected power transmission reductions for modified control strategies that incorporate constrained actuator outputs are considerably less than typical reductions with unconstrained actuator outputs.In the frequency range in which rigid body modes are present,the control strategies can only achieve 5–10 dB power transmission reduction,when control forces are constrained to be the same order of the magnitude as the primary vertical force.The resonances of the elastic mounts result in a notable increase of power transmission in high frequency range and cannot be attenuated by active control.The investigation provides a guideline for design and evaluation of active vibration isolation systems.

Objective To detect the cell viability and the expressions of stem cell surface markers after chemotherapeutic drug treatment.Methods We observed the cytotoxic effects of three chemotherapeutic agents[epirubicin(Epi),fluorouracil(5-FU)and cyclophosphamide(Cyc)]in three cell lines,and the cell viabilities after removed these chemotherapeutic agents.Expressions of stem cell surface markers CD44,CD24,CD90,CD14 and aldehyde dehydrogenase1(ALDH1)in breast cancer cells were analyzed by real-time PCR.The post hoc analysis(Tukey's tests)in conjunction with one-way ANOVA was used for statistical analysis.Results The initial cytotoxic efficacy was most notable.After the treatment of the same therapeutic agents,cell viability was decreased by 64.8%35.14%,32.25%in BT-483 cells,66.4%,22.94%and 45.88%in MDA-MB-231 cells,97.1%,99.5%and 76.4%in MCF cells.The difference was significant compared with that before treatment(P=0.000).However,the inhibitory effects were diminished after chemotherapeutic agent withdrawal.Cell viabilities were increased to 167.9%,212.04%and 188.66%in MDA-MB-231 cells at48 h after withdrawal.At 72 h after withdrawal,cell viability was increased with a significant difference in three cell lines(all P values=0.000).Expressions of CD44 and ALDH1 were most prevalent for MDA-MB-231,BT-483 and MCF-7 cells.ALDH1 mRNA level was significant higher in BT-483(HER-2 overexpression cell line)than MDA-MB-231(triple negative cell line)(P=0.012).CD14 mRNA level in MCF-7 cells were significantly lower than that in MDA-MB-231 and BT-483(P=0.003,0.001).BT-483 showed significantly higher level of CD44 than MDA-MB-231 and MCF-7 cell line(P=0.013,0.020),and no significant difference was detected between MDA-MB-231 and MCF-7 breast cancer cells(P=0.955).CD90 mRNA expressions were detected in MDA-MB-231 cells and MCF-7 cells,but not in BT-483 cells.Conclusion Some malignant cells could survive in vitro and begin to proliferate again between cycles of chemotherapy.

目的 分析肝小静脉闭塞病的临床特点、影像表现和诊治经验.方法 回顾性分析2005年1月~2011年1月我院收治的5例肝小静脉闭塞病患者的临床资料.结果 根据临床及病理学资料,将肝小静脉闭塞病分为急性期(1例)、亚急性期(1例)和慢性期(3例).CT平扫检查均发现肝脏肿大,肝实质密度降低.彩色B超检查均发现肝静脉变细,血流变缓;4例患者存在腹水.行门-体分流术3例,其中好转2例,死亡1例.行内科保守治疗2例,其中1例治愈,1例好转.结论 肝小静脉闭塞病临床表现以肝脏肿大和腹水为特征.影像学检查对肝小静脉闭塞病诊断有重要价值.急性期患者宜采取内科保守治疗,若出现门脉高压症状时,应行门-体分流术治疗.

The mass production of printed electronics can be achieved by roll-to-roll(R2R) printing system, so highly accurate web tension is required that can minimize the register error and keep the thickness and roughness of printed devices in limits. The web tension of a R2R system is regulated by the use of integrated load cells and active dancer system for printed electronics applications using decentralized multi-input-single-output(MISO) regularized variable learning rate backpropagation artificial neural networks. The active dancer system is used before printing system to reduce disturbances in the web tension of process span. The classical PID control result in tension spikes with the change in roll diameter of winder and unwinder rolls. The presence of dancer in R2R system shows that improved web tension control in printing span and the web tension can be enhanced from 3.75 N to 4.75 N. The overshoot of system is less than ±2.5 N and steady state error is within ±1 N where load cells have a signal noise of ±0.7 N. The integration of load cells and active dancer with self-adapting neural network control provide a solution to the web tension control of multispan roll-to-roll system.

目的观察临床应用克霉唑、氟康唑联合用药治疗复发性念珠菌性阴道炎的疗效.方法选择2009年2月至2010年11月收治的210例复发性念珠菌性阴道炎患者,并随机分为克霉唑组(A组)、氟康唑组(B组)和克霉唑、氟康唑联合治疗(c组)3组,每组各70例.A组患者给予克霉唑阴道片,每晚睡前阴道深部放药一粒,3d后再放1粒,连放2次;B组患者给予口服氟康唑,顿服,7d为1个治疗疗程;C组患者同时给予克霉唑和氟康唑两种药物.结果A组和B组患者均有一定的疗效,但是C组治疗效果更优于A、B两组且差异显著(P〈0.05).患者出院后回访治疗后的复发率,C组也明显低于A组和B组.3组中共有14例患者服药后出现腹泻,恶心及呕吐症状,不过症状较轻微,未采取给药措施2d后均自行痊愈.结论对于复发性念珠菌性阴道炎应用克霉唑、氟康唑联合治疗,不但疗效确切而且复发率低.

针对传统SVM主动学习中批量采样方法的不足,提出了动态可行域划分算法.从特征空间与参数空间的对偶关系入手,深入分析SVM主动学习的本质,将特征空间中对样本的标注视为参数空间中对可行域的划分;通过综合利用当前分类模型和先前标注样本两方面信息,动态地优化可行域划分方案,以确保选取的样本对模型改进的价值,最终实现更为高效的选择性采样.实验结果表明,基于动态可行域划分的SVM主动学习算法能够显著提高所选样本的信息量,从而能够在有限的标注代价下大幅提高其分类性能.