科创中国

研究放射性核素铼(188Re)标记脑胶质瘤U87-EGFRvIII(Epidermal Growth Factor Receptor Variant III)细胞反义寡核苷酸序列的制备方法,探讨其作为脑胶质瘤反义显像剂的可能性.采用细胞指数富集的配基系统进化技术(Cell-based Systematic Evolution of Ligands by Exponential Enrichment,Cell-SELEX)筛选脑胶质瘤U87-EGFRvIII细胞,得到一段高亲和力的反义寡核苷酸序列U2,然后采用直接标记法进行188Re的放射性标记,按照正交实验设计进行188Re最佳标记条件的摸索,纸层析法测定标记率,通过体外稳定性实验评价188Re-U2的放化特性,测定标记物静脉注射后在大耳兔不同时相血液放射性清除曲线,应用SPECT显像观察标记物在兔体内的放射性动态分布变化.结果表明:在最佳标记条件下188Re-U2的标记率为70%±14%;经Sep-PaK C18反相柱分离纯化后,188Re-U2在生理盐水和血清中37 oC下放置24 h的放化纯度分别为70.6%和95.55%;188Re-U2在兔体内的血放射性清除迅速,主要通过肾脏排泄,其余脏器内放射性均较低.188Re直接法标记U87-EGFRvIII细胞反义寡核苷酸U2的方法简单,具有良好的标记率和体内外稳定性,并且体内分布动力学特性也比较理想.

研究放射性核素铼(188Re)标记脑胶质瘤U87-EGFRvⅢ(Epidermal Growth Factor Receptor Variant Ⅲ)细胞反义寡核苷酸序列的制备方法,探讨其作为脑胶质瘤反义显像剂的可能性.采用细胞指数富集的配基系统进化技术(Cell-based Systematic Evolution of Ligands by Exponential Enrichment,Cell-SELEX)筛选脑胶质瘤U87-EGFRvⅢ细胞,得到一段高亲和力的反义寡核苷酸序列U2,然后采用直接标记法进行188Re的放射性标记,按照正交实验设计进行188Re最佳标记条件的摸索,纸层析法测定标记率,通过体外稳定性实验评价188Re-U2的放化特性,测定标记物静脉注射后在大耳兔不同时相血液放射性清除曲线,应用SPECT显像观察标记物在兔体内的放射性动态分布变化.结果表明:在最佳标记条件下188Re-U2的标记率为70%±14%;经Sep-PaK C18反相柱分离纯化后,188Re-U2在生理盐水和血清中37℃下放置24h的放化纯度分别为70.6%和95.55%;188Re-U2在兔体内的血放射性清除迅速,主要通过肾脏排泄,其余脏器内放射性均较低.188Re直接法标记U87-EGFRvⅢ细胞反义寡核苷酸U2的方法简单,具有良好的标记率和体内外稳定性,并且体内分布动力学特性也比较理想.

针对GJBZ188A接口导通性能测试需求,本文给出了详细的设计思路和试验数据,验证了对毫欧级导通电阻进行自动测试的可行性.导通电阻测试仪充分利用数据采集器对电压和电阻信号进行高精度测试,消除数据采集器的旁路电阻误差和通道误差,解决了快速准确地测试导通电阻的问题.导通电阻测试仪能够适应振动、高温和低温等恶劣环境,工作稳定,测试准确,已经在某型发射装置测试设备中得到应用.该测试方法简捷、实用,经过多项试验考核,验证了其可靠性和测试性,可以扩展到其它电缆导通电阻的测试.

In precision machining of complex curved surface parts with high performance, geometry accuracy is not the only constraint, but the performance should also be met. Performance of this kind of parts is closely related to the geometrical and physical parameters, so the final actual size and shape are affected by multiple source constraints, such as geometry, physics, and performance. These parts are rather difficult to be manufactured and new manufacturing method according to performance requirement is urgently needed. Based on performance and manufacturing requirements for complex curved surface parts, a new classification method is proposed, which divided the complex curved surface parts into two categories: surface re-design complex curved surface parts with multi-source constraints(PRCS) and surface unique complex curved surface parts with pure geometric constraints(PUCS). A correlation model is constructed between the performance and multi-source constraints for PRCS, which reveals the correlation between the performance and multi-source constraints. A re-design method is also developed. Through solving the correlation model of the typical part's performance-associated surface, the mapping relation between the performance-associated surface and the related removal amount is obtained. The explicit correlation model and the method for the corresponding related removal amount of the performance-associated surface are built based on the classification of surface re-design complex curved surface parts with multi-source constraints. Research results have been used in the actual processing of the typical parts such as radome, common bottom components, nozzle, et al., which shows improved efficiency and accuracy of the precision machining for the surface re-design parts with complex curved surface.

黄骅市地处河北省东部平原,地表水资源利用能力低,浅层地下水水质差,长期依赖于开采深层地下水,造成地下水环境的破坏.通过准确把握黄骅市水资源特点,合理利用地表水,最大限度利用外来水,控制利用当地地下水,保证经济社会的可持续发展.