Genealogy and phylogeography of Cyprinid fish Labeo rohita (Hamilton, 1822) inferred from ATPase 6 and 8 mitochondrial DNA gene analysis
ATPase?6/8?gene?(842?bp)?of?mitochondrial?DNA?was?sequenced?in?Labeo?rohita?samples?(n?=?253)?collected?from?nine?rivers?belonging?to?four?river?basins;?Indus,?Ganges,?Brahmaputra?and?Mahanadi.?Analysis?revealed?44?haplotypes?with?high?haplotype?diversity?(Hd)?0.694?and?low?nucleotide?diversity?(π)?0.001.?The?within?population?variation?was?larger?(83.44%)?than?among?population?differences?(16.56%).?The?mean?Fsv?value?(0.166;?P?〈?0.05)?for?overall?populations?revealed?moderate?level?of?genetic?structuring?in?the?wild?L.?rohita?populations.?The?haplotype?network?presented?a?single?clade?for?wild?L.?rohita?population,?from?different?rivers.?Negative?values?for?Fu's?index?(Fs),?mismatch?distribution?analysis?indicated?period?of?expansion?in?L.?rohita?population.?The?time?after?recent?expansion?was?estimated?for?each?population,?between?0.042?to?0.167?mya.?The?pattern?of?Isolation?by?Distance?(IBD)?was?not?significant?(r?=?-0.113,?P?〈?0.287),?when?all?the?sampling?locations?were?compared?(Mantel?test),?however,?when?an?outlier?(Indus,?Brahmaputra?and?Mahanadi)?was?removed?from?the?whole?population?set,?a?clear?positive?correlation?between?pairwise?Fsv?and?geographic?distance?(Km)?was?seen.?The?analysis?of?data?demonstrated?that?ATPase6/8?gene?polymorphism?is?a?potential?marker?to?understand?genetic?population?structure?of?wild?L.?rohita?existing?in?different?rivers.?The?study?identified?population?substructure?in?wild?L.?rohita?with?common?ancestral?origin?[Current?Zoology?60?(4):?460--471,?2014].
Rupesh K. LUHARIYA,Kuldeep K. LAL,Rajeev K. SINGH,Vindhya MOHINDRA,Arti GUPTA,Prachi MASIH,Arvind K. DWIVEDI,Rakhi DAS,U. K. CHAUHAN,J. K. JENA - 动物学报(英文版)
文章来源: 万方数据