科创中国

1"笔触"和"结构"|金秋野|黄老师,看了阿尔托的建筑,其实给我印象最深的,是那种小题大做的感觉.像他自己工作室的那个档案室(图1),这些小地方用这么大力气去经营,形成特殊的空间效果,其实是经过精心控制的.这个"人为"不是坏事,他在日用常行的基础上为建筑增加了一点更精神层面的东西.|黄居正|对一个建筑师来说,这种设计的痕迹是免不了的,即便

目的:研究单次大剂量苯巴比妥对惊厥幼鼠小脑浦肯野细胞(Purkinje cells,PCs)电生理功能的影响.方法:将健康新生7 d (P7) SD幼鼠随机分为正常对照组、惊厥模型组和苯巴比妥组.给予幼鼠腹腔注射戊四氮60 mg/kg制作惊厥模型.苯巴比妥按120 mg/kg一次性腹腔注射.采用全细胞膜片钳记录法在小脑脑片上记录小脑PCs动作电位及PCs兴奋性突触后电流(excitatory postsynaptic current,EPSC)的长时程抑制(long-term depression,LTD).结果:(1)苯巴比妥组幼鼠小脑PCs诱发动作电位的阈值降低,频率增高.(2)苯巴比妥组幼鼠小脑PCs EPSC幅值在低频刺激后15 min内降低的幅度显著大于其它2组.结论:单次大剂量苯巴比妥增加了惊厥幼鼠小脑PCs的兴奋性,并改变了小脑PCs的突触功能.

ATPase?6/8?gene?(842?bp)?of?mitochondrial?DNA?was?sequenced?in?Labeo?rohita?samples?(n?=?253)?collected?from?nine?rivers?belonging?to?four?river?basins;?Indus,?Ganges,?Brahmaputra?and?Mahanadi.?Analysis?revealed?44?haplotypes?with?high?haplotype?diversity?(Hd)?0.694?and?low?nucleotide?diversity?(π)?0.001.?The?within?population?variation?was?larger?(83.44%)?than?among?population?differences?(16.56%).?The?mean?Fsv?value?(0.166;?P?〈?0.05)?for?overall?populations?revealed?moderate?level?of?genetic?structuring?in?the?wild?L.?rohita?populations.?The?haplotype?network?presented?a?single?clade?for?wild?L.?rohita?population,?from?different?rivers.?Negative?values?for?Fu's?index?(Fs),?mismatch?distribution?analysis?indicated?period?of?expansion?in?L.?rohita?population.?The?time?after?recent?expansion?was?estimated?for?each?population,?between?0.042?to?0.167?mya.?The?pattern?of?Isolation?by?Distance?(IBD)?was?not?significant?(r?=?-0.113,?P?〈?0.287),?when?all?the?sampling?locations?were?compared?(Mantel?test),?however,?when?an?outlier?(Indus,?Brahmaputra?and?Mahanadi)?was?removed?from?the?whole?population?set,?a?clear?positive?correlation?between?pairwise?Fsv?and?geographic?distance?(Km)?was?seen.?The?analysis?of?data?demonstrated?that?ATPase6/8?gene?polymorphism?is?a?potential?marker?to?understand?genetic?population?structure?of?wild?L.?rohita?existing?in?different?rivers.?The?study?identified?population?substructure?in?wild?L.?rohita?with?common?ancestral?origin?[Current?Zoology?60?(4):?460--471,?2014].