科创中国

Most researches focused on the analytical stabilized algorithm for the modular simulation of single domain,e.g.,pure mechanical systems.Only little work has been performed on the problem of multi-domain simulation stability influenced by algebraic loops.In this paper,the algebraic loop problem is studied by a composite simulation method to reveal the internal relationship between simulation stability and system topologies and simulation unit models.A stability criterion of multi-domain composite simulation is established,and two algebraic loop compensation algorithms are proposed using numerical iteration and approximate function in multi-domain simulation.The numerical stabilized algorithm is the Newton method for the solution of the set of nonlinear equations,and it is used here in simulation of the system composed of mechanical system and hydraulic system.The approximate stabilized algorithm is the construction of response surface for inputs and outputs of unknown unit model,and it is utilized here in simulation of the system composed of forging system,mechanical and hydraulic system.The effectiveness of the algorithms is verified by a case study of multi-domain simulation for forging system composed of thermoplastic deformation of workpieces,mechanical system and hydraulic system of a manipulator.The system dynamics simulation results show that curves of motion and force are continuous and convergent.This paper presents two algorithms,which are applied to virtual reality simulation of forging process in a simulation platform for a manipulator,and play a key role in simulation efficiency and stability.

Case-Control研究表明,人线粒体基因(mtDNA) D-Loop区mt235、mt514-515、mt16183、mt16290和mt16319多态位点与有氧运动能力有关.拟从下游基因表达做进一步的研究,以探讨上述多态位点的分子调控机理.方法:采用全基因组合成法合成包含上述多态位点的DNA序列,分别连接到pGL3-promoter载体构建重组质粒,将重组质粒转染至293T细胞,观察携带5对mtDNA质粒对下游双荧光素酶报告基因的表达及荧光素酶mR-NA表达的影响.结果:携带mt235A、mt514-515CA、mt16183A和mt16319G的载体转染细胞的双荧光素酶基因表达量显著高于mt235G、mt514-515Del、mt16183C和mt16319A(P<0.01);携带mt16290两种基因型的质粒转染细胞报告基因表达没有明显差异.与pGL3-promoter载体相比,携带mt16319A的质粒转染细胞荧光素酶mRNA极显著下降(P<0.01),携带mt16290位点的两种质粒对报告基因mRNA的影响没有差异.结论:mt235、mt514-515、mt16183和mt16319多态位点影响荧光素酶基因表达,mt235A、mt514-515CA和mt16183A上调基因转录和翻译,mt16319A下调基因转录.这些基因表达上的差异可能是影响人有氧运动能力的重要调控因素.

详细介绍了SVPWM的原理.采用一种易于数字化实现的SVPWM算法实现三相电压型SVPWM整流器,该整流器采用dq坐标系下的双闭环控制.给出了PI调节器参数,以及主电路电感、电容的计算公式.在Matlab Simulink环境下搭建整流器仿真器模型.仿真结果表明,整流器能实现单位功率因数整流和能量双向流动,验证了SVPWM算法和控制系统设计方法的正确性.

本研究旨在通过茎一环RT-PCR法,建立有效定量miRNA421的PCR方法,探讨该方法在定量检测miRNA方面的应用价值.利用miRBase数据库获得miRNA-421的成熟序列.设计3条miRNA421特异性反转录引物,Q-PCR上游引物及通用下游引物.通过10倍梯度稀释RNA后特异性反转录,RT-PCR分析不同引物互相配对PCR的扩增曲线及熔融曲线,鉴定出特异性好、扩增效率高的引物.为进一步鉴定引物的有效性,过表达miRNA-d21,用鉴定的成熟引物定量扩增.利用茎一环RT-PCR法设计、鉴定出miRNA-421引物:421RT7、F19,且这对引物特异性好、扩增效率高.通过设计引物组合,茎一环RT-PCR法能够很好地用于miRNA定量分析,并具有准确、快速、节约成本的优点.