科创中国

根据化学事故应急救援与处置现场环境污染快速、实时检测需要,提出了一种化学污染物微流控芯片实时检测系统的设计方案,进行了系统的总体设计和细化设计,论述了系统设计涉及的复杂基质样品微流控芯片预处理技术,特别是固相萃取样品预处理技术、快速灵敏的微流控芯片检测技术、微流控芯片的模拟仿真、设计、集成与控制技术,以及便携式微流控芯片分析仪器的硬件设计和软件开发技术等关键技术及其研究方法,探讨了微流控芯片分离与检测单元的设计与实验、复杂基质样品的微流控芯片预处理单元设计与实验、原理性样机的研制与实验、微流控分析检测方法库和数据库的建立等系统设计的主要研究工作内容及其技术途径.通过微流控芯片分析检测系统包括样品预处理、混合、分离等单元,检测器单元,控制系统和信息处理单元的科学设计,可构建高度集成化、便携化、微型化的微流控分析检测系统,使系统具有适合小分子化合物等环境污染物的分析检测架构.

目的:探索急性早幼粒细胞白血病(acute promyelocytic leukemia,APL)中microRNA、PML-RAR α与下游靶基因组成的调控模式.方法:应用基于染色质的免疫沉淀( chromatin immunoprecipitation on chip,ChIP-on-chip)技术并结合数据分析方法对miRNA和PML- RARα与下游靶基因形成的调控环路进行研究.结果:在PML-RARα所调节的基因中,相当一部分同时也在转录后被PML-RARα潜在调节的miRNAs所调节,从而组成特定的调控环路.对于不同的内含子miRNAs,类型Ⅰ的调控环路模式在白血病细胞分化过程中更为普遍存在.结论:APL中microRNA与PML-RARα通过特定的调控环路调控下游靶基因的表达.

The electric fields employed for such work are generated using chips,such as planar linear interdigitated arrays or two parallel arrays.However,chip geometries usually affect the investigation of dielectrophoresis(DEP)and electrorotation(ER)significantly,and even may misdirect the theoretical prediction.In order to understand the electrodes geometries effect and provide a suitable range of parameters,three-dimensional simulations for the DEP and ER characterizations on the quadrupolar hyperbolical electrodes are carried out.Influences of the electrodes gaps,cell height,work region,energized voltage and frequencies for the DEP and ER manipulations are analyzed,and the analysis results show that the gaps of the electrodes and the cell height have enormous effects,but the work region is not so important.Moreover,depending on the theoretical analysis,ER experiments for polystyrene microspheres with the diameter of 20 m are carried out on two kinds of chips.The experimental results show that the microspheres rotate in the counter-field direction and the maximum rotation speed appears in the megahertz range.In addition,the experimental results are compared with the simulation results,showing that the three-dimensional simulations considering the chip geometries are more accurate than the two-dimensional predictions.This paper provides a new understanding for the theoretical predictions of DEP and ER manipulations,which decreases the difference of the theoretical and experimental results significantly,and will be significant for the lab chip research.