科创中国

用低密度聚乙烯接枝马来酸酐(LDPE-g-MAH)为原料与乙二醇缩水甘油醚(EGDE)反应合成1种新型高分子,即支链带有环氧官能目的低密度聚乙烯(LDPE-g-MAH/EGDE).研究了合成工艺和反应条件与反应程度(酸值)的关系.结果表明:反应物的质量比、催化剂用量、反应温度和时间以及溶剂用量都对酯化反应有影响.在合适的工艺条件下可以合成出浅褐色最大环氧值为0 17 mmol/g的固体产物,FT-1R和DSC光谱分析表明:此种产物为目标化合物LDPE-g-MAH/EGDE,并且LDPE-g-MAH支链上引入EGDE使其结晶能力下降.

主要探讨产品模块化设计与供应链战略协同设计问题,从产品模块化程度的考量因素分析其带来的收益及对供应链的影响,结合企业案例分析,提出产品模块化和供应链的设计决策,从而为企业模块化产品开发设计提供参考.

For the Cooperative Adaptive Cruise Control(CACC)Algorithm,existing research studies mainly focus on how inter-vehicle communication can be used to develop CACC controller,the influence of the communication delays and lags of the actuators to the string stability.However,whether the string stability can be guaranteed when inter-vehicle communication is invalid partially has hardly been considered.This paper presents an improved CACC algorithm based on the sliding mode control theory and analyses the range of CACC controller parameters to maintain string stability.A dynamic model of vehicle spacing deviation in a platoon is then established,and the string stability conditions under improved CACC are analyzed.Unlike the traditional CACC algorithms,the proposed algorithm can ensure the functionality of the CACC system even if inter-vehicle communication is partially invalid.Finally,this paper establishes a platoon of five vehicles to simulate the improved CACC algorithm in MATLAB/Simulink,and the simulation results demonstrate that the improved CACC algorithm can maintain the string stability of a CACC platoon through adjusting the controller parameters and enlarging the spacing to prevent accidents.With guaranteed string stability,the proposed CACC algorithm can prevent oscillation of vehicle spacing and reduce chain collision accidents under real-world circumstances.This research proposes an improved CACC algorithm,which can guarantee the string stability when inter-vehicle communication is invalid.

采用文献研究法,对目前我国供应链成本研究成果进行了归纳和总结,内容包括供应链成本分析工具、核算体系、影响因素、优化与控制方法、模型建立、供应链成本控制实际应用以及绿色供应链成本;通过对我国现有研究成果的分析,指出了我国供应链成本研究领域的不足以及未来的研究课题.

中年男性,贫血、高黏血症、肾功能不全、尿检阴性,免疫球蛋白lgM升高、k型lgM单克隆免疫球蛋白条带,骨髓活检提示华氏巨球蛋白血症.肾脏病理组织学见弥漫肾小管萎缩,小管基膜增厚明显;间质嗜酸性细胞浸润;肾小球病变较轻.免疫荧光染色免疫球蛋白和补体染色阴性.轻链染色显示肾小球毛细血管袢、肾小管基膜、血管壁κ轻链染色阳性.电镜下见肾小球毛细血管袢基膜内侧缘、包曼囊壁、肾小管基膜外侧缘、血管壁见泥沙样电子致密物沉积.结台临床及病理,最终诊断华氏巨球蛋白血症相关的K型轻链沉积病伴急性间质性肾炎.

为提高分散供应链计划的有效性,基于微分方程理论,建立了适合于历史数据资料、具有曲线增长特征的产品需求预测动力方程模型,并进行了实证分析.

目的 运用抗体靶向技术联合siRNA形成双特异性复合物scFv-9R-siRNA的高效递送系统,该系统通过抗OMgp的scFv把MAG,OMgp和Nogo A的特异性siRNA特异性运输至受损神经细胞,从而抑制这三种轴突抑制物的表达,改善脊髓损伤后的局部微环境,阻断这些髓磷脂相关抑制物对轴突再生的抑制作用,为后续的大鼠模型试验奠定基础.方法 (1)根据基因重组技术,利用制备好的高效抗OMgp单克隆抗体构建单链抗体scFv(OMgp),并在此基础上非共价合成针对MAG,OMgp,NogoA基因的scFv (OMgp)-9R-siRNA复合物;(2)体外鉴定scFv (OMgp)-9R-siRNA复合物效果:鉴定MAG,OMgp,NogoA三种抑制物的mRNA和蛋白表达水平,同时与非神经细胞对比,研究引入scFv后所产生的靶细胞特异性.结果 (1)成功制备抗大鼠OMgp单克隆抗体E811,并通过ELISA和Western blot对已制备的抗大鼠OMgp单克隆抗体E811进行了特异性和敏感性鉴定,在此基础上成功获得scFv (OMgp)-9R-siRNA复合物;(2)体外实验证实:scFv (OMgp)-9R-siRNA复合物同时特异性下调MAG,OMgp和Nogo A基因表达.结论 单链抗体技术(scFv)解决了siRNA的特异性导入受损神经元问题,为体内应用siRNA技术治疗脊髓损伤奠定了基础.

Objective To detect the cell viability and the expressions of stem cell surface markers after chemotherapeutic drug treatment.Methods We observed the cytotoxic effects of three chemotherapeutic agents[epirubicin(Epi),fluorouracil(5-FU)and cyclophosphamide(Cyc)]in three cell lines,and the cell viabilities after removed these chemotherapeutic agents.Expressions of stem cell surface markers CD44,CD24,CD90,CD14 and aldehyde dehydrogenase1(ALDH1)in breast cancer cells were analyzed by real-time PCR.The post hoc analysis(Tukey's tests)in conjunction with one-way ANOVA was used for statistical analysis.Results The initial cytotoxic efficacy was most notable.After the treatment of the same therapeutic agents,cell viability was decreased by 64.8%35.14%,32.25%in BT-483 cells,66.4%,22.94%and 45.88%in MDA-MB-231 cells,97.1%,99.5%and 76.4%in MCF cells.The difference was significant compared with that before treatment(P=0.000).However,the inhibitory effects were diminished after chemotherapeutic agent withdrawal.Cell viabilities were increased to 167.9%,212.04%and 188.66%in MDA-MB-231 cells at48 h after withdrawal.At 72 h after withdrawal,cell viability was increased with a significant difference in three cell lines(all P values=0.000).Expressions of CD44 and ALDH1 were most prevalent for MDA-MB-231,BT-483 and MCF-7 cells.ALDH1 mRNA level was significant higher in BT-483(HER-2 overexpression cell line)than MDA-MB-231(triple negative cell line)(P=0.012).CD14 mRNA level in MCF-7 cells were significantly lower than that in MDA-MB-231 and BT-483(P=0.003,0.001).BT-483 showed significantly higher level of CD44 than MDA-MB-231 and MCF-7 cell line(P=0.013,0.020),and no significant difference was detected between MDA-MB-231 and MCF-7 breast cancer cells(P=0.955).CD90 mRNA expressions were detected in MDA-MB-231 cells and MCF-7 cells,but not in BT-483 cells.Conclusion Some malignant cells could survive in vitro and begin to proliferate again between cycles of chemotherapy.

首先建立了一套生产型节点企业绿色度综合评价指标体系,然后综合运用熵权值法、德尔菲法、层次分析法、三角模糊函数法形成的模糊综合评价方法对绿色度的评价问题进行研究,提出了基于组合权重的三角模糊函数模糊综合评价方法及其完整的评价过程,最后以供应链上多家企业的实际数据为例进行了实证研究.

分析了我国汽车租赁行业存在的问题,提出了发挥政府在汽车租赁行业中的主导作用,建立制造商主导下的汽车租赁闭环供应链体系,实施汽车租赁连锁经营的供应链管理的汽车租赁业竞争力提升策略.