科创中国

Objective To detect the cell viability and the expressions of stem cell surface markers after chemotherapeutic drug treatment.Methods We observed the cytotoxic effects of three chemotherapeutic agents[epirubicin(Epi),fluorouracil(5-FU)and cyclophosphamide(Cyc)]in three cell lines,and the cell viabilities after removed these chemotherapeutic agents.Expressions of stem cell surface markers CD44,CD24,CD90,CD14 and aldehyde dehydrogenase1(ALDH1)in breast cancer cells were analyzed by real-time PCR.The post hoc analysis(Tukey's tests)in conjunction with one-way ANOVA was used for statistical analysis.Results The initial cytotoxic efficacy was most notable.After the treatment of the same therapeutic agents,cell viability was decreased by 64.8%35.14%,32.25%in BT-483 cells,66.4%,22.94%and 45.88%in MDA-MB-231 cells,97.1%,99.5%and 76.4%in MCF cells.The difference was significant compared with that before treatment(P=0.000).However,the inhibitory effects were diminished after chemotherapeutic agent withdrawal.Cell viabilities were increased to 167.9%,212.04%and 188.66%in MDA-MB-231 cells at48 h after withdrawal.At 72 h after withdrawal,cell viability was increased with a significant difference in three cell lines(all P values=0.000).Expressions of CD44 and ALDH1 were most prevalent for MDA-MB-231,BT-483 and MCF-7 cells.ALDH1 mRNA level was significant higher in BT-483(HER-2 overexpression cell line)than MDA-MB-231(triple negative cell line)(P=0.012).CD14 mRNA level in MCF-7 cells were significantly lower than that in MDA-MB-231 and BT-483(P=0.003,0.001).BT-483 showed significantly higher level of CD44 than MDA-MB-231 and MCF-7 cell line(P=0.013,0.020),and no significant difference was detected between MDA-MB-231 and MCF-7 breast cancer cells(P=0.955).CD90 mRNA expressions were detected in MDA-MB-231 cells and MCF-7 cells,but not in BT-483 cells.Conclusion Some malignant cells could survive in vitro and begin to proliferate again between cycles of chemotherapy.

目的利用AAV介导的体细胞基因敲除技术,在人大肠癌细胞系HCTll6中对靶基因Sirt1进行敲除,达到基因沉默的目的.方法构建Sirtl打靶载体,通过病毒包装、感染、药物筛选、PCR鉴定、Westernblotting鉴定等步骤获得Sirt1基因敲除的细胞株.结果经过筛选和鉴定后获得两个Sirtl-/-阳性克隆,成功构建HCTll6Sirtl.一细胞系.结论通过体细胞敲除技术,我们成功地获得Sirtl敲除肠癌细胞株.

鉴于南水北调中线工程线路选择的位置对工程安全、水质保护、投资等方面影响较大,虽然南水北调中线工程总干渠线路的选择经过了几十年的论证,在总体线路方案已经确定的情况下,仍需开展局部线路论证工作并对其进行调整与优化.现以十里河段为例对局部线路的调整与优化进行了分析与研究.在开展十里河段线路优化工作阶段,经过现场查勘并考虑地形、地质条件及村镇分布等情况,初步选择了深挖方的北线方案及高填方的南线方案,并对北线、南线两个方案开展了有关工程安全、投资、技术等方丽的分析与研究工作,最终提出了技术可行、经济合理的输水线路布置方案.

Straight-line compliant mechanisms are important building blocks to design a linear-motion stage,which is very useful in precision applications.However,only a few configurations of straight-line compliant mechanisms are applicable.To construct more kinds of them,an approach to design large-displacement straight-line flexural mechanisms with rotational flexural joints is proposed,which is based on a viewpoint that the straight-line motion is regarded as a compromise of rigid and compliant parasitic motion of a rotational flexural joint.An analytical design method based on the Taylor series expansion is proposed to quickly obtain an approximate solution.To illustrate and verify the proposed method,two kinds of flexural joints,cross-axis hinge and leaf-type isosceles-trapezoidal flexural(LITF)pivot are used to reconstruct straight-line flexural mechanisms.Their performances are obtained by analytic and FEA method respectively.The comparisons of the results show the accuracy of the approach.Both examples show that the proposed approach can convert a large-deflection flexural joint into approximate straight-line mechanism with a high linearity that is higher than 5 000within 5 mm displacement.This can lead to a new way to design,analyze or optimize straight-line flexure mechanisms.

报告1例透明细胞汗腺瘤.患者女,22岁.3年前无意中发现左下腹一核桃大褐色半透明状实性肿块,逐渐增大,现约3.2cm*1.6cm*1.3cm,有蒂,表面光滑,质地中等,无压痛感.

根据金沟河涡管排沙工程的布置形式及排沙要求,提出了排沙涡管开口宽度、涡管直径、涡管与渠道轴线夹角、涡管开口长度、涡管纵向坡度等技术指标的确定方法,计算并分析了排沙涡管截沙率及排沙效果.结果表明:通过对排沙涡管进行改进,金沟河排沙平均耗水量占引进水量的4.51%,符合原设计的技术指标.

官厅水库是首都北京的备用水源地,在分析官厅流域水功能区2009年水环境状况基础上,根据污染物入河量,建立官厅流域水功能区限制纳污红线,按照2015年、2020年控制目标,把限制纳污红线指标细化分解到各个排污口,为限制纳污红线的实施提供更加切实可行的技术支撑.

通过对工业用接线端子台顶端本体零件的工艺分析,按照塑料模具设计的相关原则,从分型面选择、浇注系统设计、抽芯机构设计、冷却系统设计、到模具零件的设计和加工工艺流程,实现了该复杂零件的一模两腔模具设计结构.

The mass production of printed electronics can be achieved by roll-to-roll(R2R) printing system, so highly accurate web tension is required that can minimize the register error and keep the thickness and roughness of printed devices in limits. The web tension of a R2R system is regulated by the use of integrated load cells and active dancer system for printed electronics applications using decentralized multi-input-single-output(MISO) regularized variable learning rate backpropagation artificial neural networks. The active dancer system is used before printing system to reduce disturbances in the web tension of process span. The classical PID control result in tension spikes with the change in roll diameter of winder and unwinder rolls. The presence of dancer in R2R system shows that improved web tension control in printing span and the web tension can be enhanced from 3.75 N to 4.75 N. The overshoot of system is less than ±2.5 N and steady state error is within ±1 N where load cells have a signal noise of ±0.7 N. The integration of load cells and active dancer with self-adapting neural network control provide a solution to the web tension control of multispan roll-to-roll system.